• Catalog Number:

  E1A7007A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of Serine 485/491(S-G- SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13131 P54646

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at Serine 485 or Serine 491.

• References:

  Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Immunohistochemical analysis of paraffin- embedded colon carcinoma, using AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody.Line1: The extracts from HeLa cells; Line2: The extracts from HeLa cells treated with heat shock.