• Catalog Number:

  E1A7005A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Threonine 308 (M-K-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  Akt(Phospho-Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Threonine 308.

• References:

  Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84. Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23. Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73. Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Akt(Phospho-Thr308) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis using Akt(Phospho-Thr308) Antibody: Line1: The extracts from 293 cells untreated; Line2: The extracts from 293 cells treated with insulin.