• Catalog Number:

  E1A7004A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Serine473 (Q-F-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  Akt(Phospho-Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Serine 473.

Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.

Min YH, et al. (2004) Cancer Res; 64(15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using Akt (Phospho-Ser473) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.