• Catalog Number:

  E1A1243A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P08670

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  VIME, vimentin

• Specificity Sensitivity:

  Vimentin (Phospho-Ser56) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 56.

• References:

  Ota T., Nat. Genet. 36:40-45(2004). Rasmussen R.K., Electrophoresis 18:588-598(1997). Gupta A.K., Gene 86:303-304(1990). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser56

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho-Ser56) antibody. Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Phospho-Ser56) antibody.