• Catalog Number:

  E1A1241A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VAV2 around the phosphorylation site of tyrosine 142 (D-V-YP-R-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P52735

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Vav-2 protein

• Specificity Sensitivity:

  VAV2 (Phospho-Tyr142) antibody detects endogenous levels of VAV2 only when phosphorylated at tyrosine 142.

• References:

  Henske E.P., Ann. Hum. Genet. 59:25-37(1995). Tamas P., J. Biol. Chem. 278:5163-5171(2003). RIKEN structural genomics initiative (RSGI); Submitted (APR-2007) to the PDB data bank.

• All Sites:

  Human: Ser142; Mouse: Ser142

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using VAV2 (Phospho-Tyr142) antibody. Western blot analysis of extracts from K562 cells and HeLa cells, treated with TNF (200ng/ml, 30mins), using VAV2 (Phospho-Tyr142) antibody.

P-peptide - + Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) antibody.