• Catalog Number:

  E1A1239A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Telomeric Repeat Binding Factor 1 around the phosphorylation site of serine 219 (I-I-SP-Q-K).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P54274

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  NIMA-interacting protein 2; PIN2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor 1; TERF1; TRF; TTAGGG repeat binding factor 1

• Specificity Sensitivity:

  Telomeric Repeat Binding Factor 1 (Phospho-Ser219) antibody detects endogenous levels of Telomeric Repeat Binding Factor 1 only when phosphorylated at serine 219.

• References:

  Chong L., Science 270:1663-1667(1995). Broccoli D., Nat. Genet. 17:231-235(1997). Shen M., Proc. Natl. Acad. Sci. U.S.A. 94:13618-13623(1997).

• All Sites:

  Human: Ser219

Lane 1: The extracts from 293 cells treated with paclitaxel (1uM, 24hours) using Telomeric Repeat Binding Factor 1 (Phospho-Ser219) antibody Lane 2: The extracts from 293 cells treated with paclitaxel (1uM, 24hours) plus phosphopeptide using Telomeric Repeat Binding Factor 1 (Phospho-Ser219) antibody Lane 3: The extracts from293 cells treated with paclitaxel (1uM, 24hours) using Telomeric Repeat Binding Factor 1 (Ab-219) antibody