Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human SNAI1 around the phosphorylation site of serine 246 (T-F-SP-R-M).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
O95863
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
Other Names:
SNAH, SNAI, Sna, Snail, zinc finger protein SNAI1n
Specificity Sensitivity:
SNAI1 (Phospho-Ser246) antibody detects endogenous levels of SNAI1 only when phosphorylated at serine 246.
P-peptide - +
Western blot analysis of extracts from HT29 cells, using SNAI1 (Phospho-Ser246) antibody.
Immunofluorescence analysis of HuvEc cells, using SNAI1 (Phospho-Ser246) antibody.