• Catalog Number:

  E1A1233A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of serine 885 (R-R-SP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8TDA3

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  GEF-H1, GEFH1, Guanine nucleotide exchange factor GEF-H1, proliferating cell nucleolar antigen p40, rho/rac guanine nucleotide exchange factor 2

• Specificity Sensitivity:

  Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody detects endogenous levels of Rho/Rac Guanine Nucleotide Exchange Factor 2 only when phosphorylated at serine 885.

• References:

  Ren Y., J. Biol. Chem. 273:34954-34960(1998). Krendel M., Nat. Cell Biol. 4:294-301(2002). Ishikawa K., DNA Res. 5:169-176(1998).

• All Sites:

  Human: Ser885; Mouse: Ser885

Western blot analysis of extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin(40nM, 30mins), using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) antibody.