• Catalog Number:

  E1A1220A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFAT5 around the phosphorylation site of serine 1197 (M-L-SP-Q-E).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 90%)

• SwissProtNo:

  O94916

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  KIAA0827, NF-AT5, NFAT5, T cell transcription factor NFAT5, TonE-binding protein, nuclear factor of activated T cells 5, tonicity-responsive enhancer-binding protein

• Specificity Sensitivity:

  NFAT5 (Phospho-Ser1197) antibody detects endogenous levels of NFAT5 only when phosphorylated at serine 1197.

• References:

  Jonathan H. Esensten, Nucleic Acids Res., Jul 2005; 33: 3845 - 3854. Jason Trama, J. Immunol., Nov 2002; 169: 5477. Cristina López-Rodríguez, PNAS, Feb 2004; 101: 2392.

• All Sites:

  Human: Ser1197; Mouse: Ser1197

P-peptide - +

Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) antibody. NFAT5 (Phospho-Ser1197) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.