Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human KIF2C around the phosphorylation site of serine 95 (R-R-SP-V-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
Q99661
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
KF2C, KIF2C, KNSL6, Kinesin-like protein 6, Kinesin-like protein KIF2C, Mitotic centromere-associated kinesin
Specificity Sensitivity:
KIF2C (Phospho-Ser95) antibody detects endogenous levels of KIF2C only when phosphorylated at serine 95.
References:
Kim I.-G., Biochim. Biophys. Acta 1359:181-186(1997).
Cheng L.J.; Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
All Sites:
Human: Ser95; Mouse: Ser92
P-peptide - +
Western blot analysis of extracts from HeLa cells, treated with TNF (10ng/ml, 30mins), using KIF2C (Phospho-Ser95) antibody.
Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) antibody.