Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human IGF2R around the phosphorylation site of serine 2409 (Q-D-SP-E-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
IGF2R (Phospho-Ser2409) antibody detects endogenous levels of IGF2R only when phosphorylated at serine 2409.
References:
Morgan D.O., Nature 329:301-307(1987).
Oshima A., J. Biol. Chem. 263:2553-2562(1988).
Killian J.K., Mamm. Genome 10:74-77(1999).
All Sites:
Human: Ser2409; Mouse: Ser2401
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGF2R (Phospho-Ser2409) antibody.
Western blot analysis of extracts from COS-7 cells, treated with UV (15mins), using IGF2R (Phospho-Ser2409) antibody.