Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FOXL2 around the phosphorylation site of serine 263 (V-Q-SP-M-A).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P58012
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Other Names:
BPES, BPES1, PFRK, PINTO, POF3, blepharophimosis, epicanthus inversus, and ptosis 1, forkhead box protein L2
Specificity Sensitivity:
FOXL2 (Phospho-Ser263) antibody detects endogenous levels of FOXL2 only when phosphorylated at serine 263.
References:
Crisponi L., Nat. Genet. 27:159-166(2001).
Xu Y.; Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
All Sites:
Human: Ser263
Western blot analysis of extracts from K562 cells, treated with Na3VO4 (0.3nM, 40mins), using FOXL2 (Phospho-Ser263) antibody.