• Catalog Number:

  E1A1196A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Desmin around the phosphorylation site of serine 60 (R-T-SP-G-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P17661

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  DES, DESM

• Specificity Sensitivity:

  Desmin (Phospho-Ser60) antibody detects endogenous levels of Desmin only when phosphorylated at serine 60.

• References:

  H Li. J. Cell Biol., Mar 1994; 124: 827. G. A. Ramm. Am J Physiol Gastrointest Liver Physiol, Oct 1995; 269: 532. M Vitadello, J. Cell Sci., Sep 1990; 97: 11.

• All Sites:

  Human: Ser60; Mouse: Ser60; Rat: Ser60

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Desmin (Phospho-Ser60) antibody. Desmin (Phospho-Ser60) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.