• Catalog Number:

  E1A1192A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Casein Kinase II α around the phosphorylation site of tyrosine 255 (D-L-YP-D-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P68400

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  Casein kinase II, alpha chain; CK II; CK2-alpha1; CK2A1; CKIIA; CSK21; CSNK2A1; EC 2.7.11.1; kinase CK2-alpha1

• Specificity Sensitivity:

  Casein Kinase II α (Phospho-Tyr255) antibody detects endogenous levels of Casein Kinase II α only when phosphorylated at tyrosine 255.

• References:

  Meisner H., Biochemistry 28:4072-4076(1989). Lozeman F.J., Biochemistry 29:8436-8447(1990). Deloukas P., Nature 414:865-871(2001).

• All Sites:

  Human: Tyr255; Mouse: Tyr255; Rat: Tyr255

Western blot analysis of extracts from HeLa cells and Jurkat cells, using Casein Kinase II α (Phospho-Tyr255) antibody.