• Catalog Number:

  E1A1189A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ARHGDIA around the phosphorylation site of serine 174 (R-G-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P52565

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  GDIR, Rho GDI 1, Rho GDP-dissociation inhibitor 1, Rho-GDI alpha

• Specificity Sensitivity:

  ARHGDIA (Phospho-Ser174) antibody detects endogenous levels of ARHGDIA only when phosphorylated at serine 174.

• References:

  Leffers H., Exp. Cell Res. 209:165-174(1993). Maeda A., Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases. Chuang T.H., Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser174; Mouse: Ser174; Rat: Ser174

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using ARHGDIA (Phospho-Ser174) antibody. Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using ARHGDIA (Phospho-Ser174) antibody.