• Catalog Number:

  E1A1186A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human WEE1 around the phosphorylation site of serine 642 (S-V-SP-L-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P30291

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  EC 2.7.10.2; kinase WEE1; Wee1-like protein kinase; WEE1A; WEE1hu

• Specificity Sensitivity:

  WEE1 (Phospho-Ser642) antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.

• References:

  Watanabe N., EMBO J. 14:1878-1891(1995). Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001). Igarashi M., Nature 353:80-83(1991).

• All Sites:

  Human: Ser642; Mouse: Ser642; Rat: Ser642

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 60mins), using WEE1 (Phospho-Ser642) antibody.