• Catalog Number:

  E1A1181A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SSB around the phosphorylation site of serine 366 (F-A-SP-D-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05455

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  La antigen, La autoantigen, La ribonucleoprotein, Lupus La protein, SS-B, Sjogren syndrome type B antigen

• Specificity Sensitivity:

  SSB (Phospho-Ser366) antibody detects endogenous levels of SSB only when phosphorylated at serine 366.

• References:

  Fan H., Cell 88:707-715(1997). Fouraux M.A., RNA 8:1428-1443(2002). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Ser366

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using SSB (Phospho-Ser366) antibody. Western blot analysis of extracts from 293 cells, using SSB (Phospho-Ser366) antibody.

Forskolin + - Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using SSB (Phospho-Ser366) antibody.