• Catalog Number:

  E1A1177A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human POU2F1/OCT1 around the phosphorylation site of serine385 (R-T-SP-I-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P14859/P09086

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  NF-A1; OCT1; OTF-1; Octamer-binding transcription factor 1; PO21; PO2F1; POU domain, class 2; transcription factor 1; POU2F1

• Specificity Sensitivity:

  POU2F1/OCT1 (Phospho-Ser385) antibody detects endogenous levels of POU2F1/OCT1 only when phosphorylated at serine 385.

• References:

  Sturm R.A., Genes Dev. 2:1582-1599(1988). Luchina N.N., Immunol. Lett. 85:237-241(2003). Gregory S.G., Nature 441:315-321(2006).

• All Sites:

  Human: Ser385; Mouse: Ser388; Rat: Ser274

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using POU2F1/OCT1 (Phospho-Ser385) antibody.