• Catalog Number:

  E1A1175A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457 (F-V-SP-E-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q53EL6

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  neoplastic transformation inhibitor; nuclear antigen H731-like; programmed cell death protein 4

• Specificity Sensitivity:

  PDCD4 (Phospho-Ser457) antibody detects endogenous levels of PDCD4 only when phosphorylated at serine 457.

• References:

  Matsuhashi S., Res. Commun. Biochem. Cell Mol. Biol. 1:109-120(1997). Azzoni L., J. Immunol. 161:3493-3500(1998). Deloukas P., Nature 429:375-381(2004).

• All Sites:

  Human: Ser457; Mouse: Ser457; Rat: Ser457

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PDCD4 (Phospho-Ser457) antibody. Western blot analysis of extracts from 293 cells, treated with serum (20%, 15mins), using PDCD4 (Phospho-Ser457) antibody.