• Catalog Number:

  E1A1171A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 370 (R-P-SP-A-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P14598

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox

• Specificity Sensitivity:

  p47 phox (Phospho-Ser370) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 370.

• References:

  PL Bunger, J. Leukoc. Biol., Jan 2000; 67: 63. J Rosenthal, Blood, Dec 1996; 88: 4321 - 4329. Sen-Lin Li, J. Biol. Chem., Jul 1997; 272: 17802.

• All Sites:

  Human: Ser370; Mouse: Ser370; Rat: Ser369

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using p47 phox (Phospho-Ser370) antibody. p47 phox (Phospho-Ser370) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.