• Catalog Number:

  E1A1168A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of serine 351 (L-P-SP-K-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P22736

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Early response protein NAK1, GFRP, GFRP1, HMR, N10, N10 nuclear protein, NAK1, NGFI-B, NGFIB, NR4A1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, TR3 orphan receptor

• Specificity Sensitivity:

  Nuclear Receptor NR4A1 (Phospho-Ser351) antibody detects endogenous levels of Nuclear Receptor NR4A1 only when phosphorylated at serine 351.

• References:

  Nakai A., Mol. Endocrinol. 4:1438-1443(1990). Chang C., J. Steroid Biochem. 34:391-395(1989). Bondy G.P., Cell Growth Differ. 2:203-208(1991). - ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Ser351; Mouse: Ser354; Rat: Ser350

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody. Nuclear Receptor NR4A1 (Phospho-Ser351) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.