• Catalog Number:

  E1A1166A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 234 (E-K-TP-P-K).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06748

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin

• Specificity Sensitivity:

  NPM (Phospho-Thr234) antibody detects endogenous levels of NPM only when phosphorylated at threonine 234.

• References:

  Chan W.-Y., Biochemistry 28:1033-1039(1989). Li X., Biochem. Biophys. Res. Commun. 163:72-78(1989). Zhang X.T., Biochem. Biophys. Res. Commun. 164:176-184(1989).

• All Sites:

  Human: Thr234; Mouse: Thr232; Rat: Thr232

Western blot analysis of extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours), using NPM (Phospho-Thr234) antibody.