Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NIPA around the phosphorylation site of serine 354 (T-R-SP-W-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q86WB0
Applications:
WB: 1:500~1:1000 ELISA: 1:40000
Other Names:
Nuclear interacting partner of ALK, Nuclear-interacting partner of ALK, ZC3HC1
Specificity Sensitivity:
NIPA (Phospho-Ser354) antibody detects endogenous levels of NIPA only when phosphorylated at serine 354.
References:
Ouyang T., J. Biol. Chem. 278:30028-30036(2003).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
Ota T., Nat. Genet. 36:40-45(2004).
All Sites:
Human: Ser354; Mouse: Ser353; Rat: Ser353
Western blot analysis of extracts from COS7 cells, treated with HU (2nM, 24hours), using NIPA (Phospho-Ser354) antibody.