• Catalog Number:

  E1A1160A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of serine 304 (R-S-SP-I-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P14598

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox

• Specificity Sensitivity:

  Neutrophil Cytosol Factor 1 (Phospho-Ser304) antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304.

• References:

  Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990). Gorlach A., J. Clin. Invest. 100:1907-1918(1997). Chanock S.J., Blood Cells Mol. Dis. 26:37-46(2000).

• All Sites:

  Human: Ser304

Western blot analysis of extracts from COS7 cells treated with UV (15mins) (line 1) and A549 cells (line 2), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) antibody.