• Catalog Number:

  E1A1159A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NEK9 around the phosphorylation site of threonine 210(A-E-TP-L-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8TD19

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  EC 2.7.11.1, KIAA1995, NERCC, NIMA-related kinase 8, Nek8, Nercc1 kinase, NimA-related protein kinase 9, Serine/threonine-protein kinase Nek9

• Specificity Sensitivity:

  NEK9 (Phospho-Thr210) antibody detects endogenous levels of NEK9 only when phosphorylated at threonine 210.

• References:

  Holland P.M., J. Biol. Chem. 277:16229-16240(2002). Roig J., Genes Dev. 16:1640-1658(2002). Ohara O., DNA Res. 9:47-57(2002).

• All Sites:

  Human: Thr210; Mouse: Thr210

P-peptide - + Western blot analysis of extracts from HepG2 cells, using NEK9 (Phospho-Thr210) antibody. Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) antibody.