• Catalog Number:

  E1A1156A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Mtor around the phosphorylation site of threonine 2446 (T-R-TP-D-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42345

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR

• Specificity Sensitivity:

  Mtor (Phospho-Thr2446) antibody detects endogenous levels of Mtor only when phosphorylated at threonine 2446.

• References:

  Brown E.J., Nature 369:756-758(1994). Onyango P., Genomics 50:187-198(1998). Gregory S.G., Nature 441:315-321(2006).

• All Sites:

  Human: Thr2446; Mouse: Thr2446; Rat: Thr2446

Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using Mtor (Phospho-Thr2446) antibody.