• Catalog Number:

  E1A1145A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29474

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Constitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS

• Specificity Sensitivity:

  eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.

• References:

  Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). Janssens S.P., J. Biol. Chem. 267:22694-22694(1992). Marsden P.A., FEBS Lett. 307:287-293(1992).

• All Sites:

  Human: Ser615; Mouse: Ser614; Rat: Ser614

Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.