Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human TGF β Receptor I around the phosphorylation site of serine 165 (D-P-SP-L-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P36897
Applications:
IF: 1:100~1:500 ELISA: 1:5000
Other Names:
ADRB2; ALK-5; Activin receptor-like kinase 5; ESK2; SKR4; TGF-beta receptor type I precursor; TGF-beta type I receptor; TGF-betaR1; TGFR-1; TGFR1; kinase TGF-beta-R1; receptor R4
Specificity Sensitivity:
TGF β Receptor I (Phospho-Ser165) antibody detects endogenous levels of TGF β Receptor I only when phosphorylated at serine 165.
References:
Franzen P., Cell 75:681-692(1993).
Vellucci V.F., Genomics 46:278-283(1997).
Lynch M.A., Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
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All Sites:
Human: Ser165; Mouse: Ser165; Rat: Ser163
P-peptide - +
Immunofluorescence analysis of HeLa cells, using TGF β Receptor I (Phospho-Ser165) antibody.