• Catalog Number:

  E1A1122A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4/JNKK1 around the phosphorylation site of serine 257 (V-D-SP-I-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45985

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  C-JUN N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC 2.7.12.2, JNK activating kinase 1, JNK kinase 1, JNKK, JNKK 1, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MKK4, MP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1, c-Jun N- terminal kinase kinase 1, kinase SEK1

• Specificity Sensitivity:

  SEK1/MKK4/JNKK1 (Phospho-Ser257) antibody detects endogenous levels of SEK1/MKK4/JNKK1 only when phosphorylated at serine 257.

• References:

  Barbara A. Yoshida, Cancer Res., Nov 1999; 59: 5483 - 5487. S. Diane Yamada, Cancer Res., Nov 2002; 62: 6717 - 6723. Soula Ganiatsas, PNAS, Jun 1998; 95: 6881. Jonathan A. Hickson, Cancer Res., Feb 2006; 66: 2264 - 2270.

• All Sites:

  Human: Ser257; Mouse: Ser255; Rat: Ser135

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using SEK1/MKK4/JNKK1 (Phospho-Ser257) antibody.