• Catalog Number:

  E1A1119A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104 (K-F-YP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P43351

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  DNA repair protein RAD52 homolog, RA52

• Specificity Sensitivity:

  RAD52 (Phospho-Tyr104) antibody detects endogenous levels of RAD52 only when phosphorylated at tyrosine 104.

• References:

  Shen Z., Genomics 25:199-206(1995). Muris D.F., Mutat. Res. 315:295-305(1994). Matsuoka S., Science 316:1160-1166(2007).

• All Sites:

  Human: Tyr104; Mouse: Tyr105

Western blot analysis of extracts from COS cells, treated with H2O2 (100uM, 30mins), using RAD52 (Phospho-Tyr104) antibody.