• Catalog Number:

  E1A1117A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RAD51 around the phosphorylation site of tyrosine 315 (K-I-YP-D-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q06609

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  DNA repair protein RAD51 homolog 1, RAD51A, RECA

• Specificity Sensitivity:

  RAD51 (Phospho-Tyr315) antibody detects endogenous levels of RAD51 only when phosphorylated at tyrosine 315.

• References:

  Shinohara A., Nat. Genet. 4:239-243(1993). Yoshimura Y., Nucleic Acids Res. 21:1665-1665(1993). Schmutte C., Cancer Res. 59:4564-4569(1999). R.E., Daly M., Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001).

• All Sites:

  Human: Tyr315; Mouse: Tyr315

Western blot analysis of extracts from Jurkat cells, using RAD51 (Phospho-Tyr315) antibody.