Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PAK1/2 around the phosphorylation site of serine 199 (T-K-SP-V-Y).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PAK1/2 (Phospho-Ser199) antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.
References:
Brown J.L., Curr. Biol. 6:598-605(1996).
Sells M.A., Curr. Biol. 7:202-210(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
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All Sites:
Human: Ser199; Mouse: Ser199; Rat: Ser198
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody.
Western blot analysis of extracts from LOVO cells, treated with starved (24hours), using PAK1/2 (Phospho-Ser199) antibody.