• Catalog Number:

  E1A1106A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NIFK around the phosphorylation site of threonine 234 (G-P-TP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8TB66

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  MKI67IP, NIFK, Nucleolar protein NIFK, RNA binding protein

• Specificity Sensitivity:

  NIFK (Phospho-Thr234) antibody detects endogenous levels of NIFK only when phosphorylated at threonine 234.

• References:

  J G Li, J. Bacteriol., Oct 1990; 172: 5884 - 5891. Masatoshi Takagi, J. Biol. Chem., Jun 2001; 276: 25386 - 25391. AM Hirsch, Mol. Biol. Evol., Jan 1995; 12: 16 - 27. J G Li, J. Bacteriol., Oct 1990; 172: 5884 - 5891.

• All Sites:

  Human: Thr234; Mouse: Thr257

Peptide - +

Peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using NIFK (Phospho-Thr234) antibody. Immunofluorescence analysis of HuvEc cells, using NIFK (Phospho-Thr234) antibody.