• Catalog Number:

  E1A1097A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MCL1 around the phosphorylation site of serine 159 (D-G-SP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 77%, Positives = 77%)

• SwissProtNo:

  Q07820

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  EAT/MCL-1 protein, Induced myeloid leukemia cell differentiation protein Mcl-1, Myeloid cell leukemia sequence 1

• Specificity Sensitivity:

  MCL1 (Phospho-Ser159) antibody detects endogenous levels of MCL1 only when phosphorylated at serine 3159.

• References:

  Alfredo De Biasio, J. Biol. Chem., Jun 2007; 10.1074/jbc.M700938200. Potchanapond Graidist, J. Biol. Chem., Sep 2004; 279: 40868 - 40875. Kenichi Fujise, J. Biol. Chem., Dec 2000; 275: 39458.

• All Sites:

  Human: Ser159; Mouse: Ser159

P-peptide - +

Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) antibody. MCL1 (Phospho-Ser159) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.