Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MARK1/2/3/4 around the phosphorylation site of threonine 215 (L-D-TP-F-C).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q9P0L2/Q7KZI7/P27448/Q96L34
Applications:
IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
MAP/microtubule affinity-regulating kinase 1, MARK
Specificity Sensitivity:
MARK1/2/3/4 (Phospho-Thr215) antibody detects endogenous levels of MARK1/2/3/4 only when phosphorylated at threonine 215.
References:
Lizcano J.M., EMBO J. 23:833-843(2004).
Zhou H.J., Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Nagase T., DNA Res. 7:143-150(2000).