• Catalog Number:

  E1A1091A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAD1 around the phosphorylation site of serine 428 (E-Y-SP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q9Y6D9

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  HsMAD1, MAD1, MAD1-like 1, MD11, MD1L1, Mitotic arrest deficient-like protein 1, Mitotic checkpoint MAD1 protein-homolog, Mitotic spindle assembly checkpoint protein MAD1, TXBP181, Tax binding protein-181, Hmad1

• Specificity Sensitivity:

  MAD1 (Phospho-Ser428) antibody detects endogenous levels of MAD1 only when phosphorylated at serine 428.

• References:

  Stefanie Gehring, J. Biol. Chem., Mar 2000; 275: 10413. Brian J. Tunquist, J. Cell Biol., Dec 2003; 163: 1231. Yoichi Iwanaga, J. Biol. Chem., Aug 2002; 277: 31005 - 31013.

• All Sites:

  Human: Ser428

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MAD1 (Phospho-Ser428) antibody. MAD1 (Phospho-Ser428) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.