• Catalog Number:

  E1A1090A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LEF-1 around the phosphorylation site of serine 42 (E-I-SP-H-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UJU2

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

• Specificity Sensitivity:

  LEF-1 (Phospho-Ser42) antibody detects endogenous levels of LEF-1 only when phosphorylated at serine 42.

• References:

  Waterman M.L., Genes Dev. 5:656-669(1991). Hovanes K., Nucleic Acids Res. 28:1994-2003(2000). Kobielak A., Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser42; Mouse: Ser40; Rat: Ser40

Western blot analysis of extracts from 293 cells, treated with paclitaxel (1uM, 24hours), using LEF-1 (Phospho-Ser42) antibody.