• Catalog Number:

  E1A1083A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Kir5.1 around the phosphorylation site of serine 417 (M-E-SP-Q-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Mouse, Rat

• SwissProtNo:

  Q9NPI9

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  IRK16, IRKG, Inward rectifier K channel Kir5.1, KCNJ16, Potassium channel, inwardly rectifying, subfamily J, member 16

• Specificity Sensitivity:

  Kir5.1 (Phospho-Ser417) antibody detects endogenous levels of Kir5.1 only when phosphorylated at serine 417.

• References:

  Liu Y., Cytogenet. Cell Genet. 90:60-63(2000). Derst C., FEBS Lett. 491:305-311(2001).

• All Sites:

  Mouse: Ser417; Rat: Ser417

Western blot analysis of extracts from RAW264.7 cells, treated with forskolin (40nM, 30mins), using Kir5.1 (Phospho-Ser417) antibody.