• Catalog Number:

  E1A1081A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human KIF11/Eg5 around the phosphorylation site of threonine 926 (G-T-TP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P52732

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  Eg5, KIFL1, KNSL1, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related mitotic motor protein, Kinesin-related motor protein Eg5, TRIP5, Thyroid receptor interacting protein 5

• Specificity Sensitivity:

  KIF11/Eg5 (Phospho-Thr926) antibody detects endogenous levels of KIF11/Eg5 only when phosphorylated at threonine 926.

• References:

  Blangy A., Cell 83:1159-1169(1995). Whitehead C.M., J. Cell Sci. 111:2551-2561(1998). Hunt S.E., Nature 429:375-381(2004). Lee J.W., Mol. Endocrinol. 9:243-254(1995).

• All Sites:

  Human: Thr926

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using KIF11/Eg5 (Phospho-Thr926) antibody. Western blot analysis of extracts from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 (Phospho-Thr926) antibody.