• Catalog Number:

  E1A1066A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IL-8Rβ/CDw128β around the phosphorylation site of serine 347 (R-P-SP-F-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P25025

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  CDw128b, CXCR-2, CXCR2, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8 receptor type 2, IL8RB, interleukin-8 receptor B

• Specificity Sensitivity:

  IL-8Rβ/CDw128β (Phospho-Ser347) antibody detects endogenous levels of IL-8Rβ/CDw128β only when phosphorylated at serine 347.

• References:

  Murphy P.M., Science 253:1280-1283(1991). Cerretti D.P., Mol. Immunol. 30:359-367(1993). Ahuja S.K., J. Biol. Chem. 269:26381-26389(1994).

• All Sites:

  Human: Ser347; Mouse: Ser346

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using IL-8Rβ/CDw128β (Phospho-Ser347) antibody. Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using IL-8Rβ/CDw128β (Phospho-Ser347) antibody.

P-peptide - + Immunofluorescence analysis of COS7 cells, using IL-8Rβ/CDw128β (Phospho-Ser347) antibody.