• Catalog Number:

  E1A1052A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IGFBP-3 around the phosphorylation site of serine 183 (K-D-SP-Q-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 90%)

• SwissProtNo:

  P17936

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  IBP-3, IBP3, IGF-binding protein 3, IGFBP3, Insulin-like growth factor binding protein 3 precursor

• Specificity Sensitivity:

  IGFBP-3 (Phospho-Ser183) antibody detects endogenous levels of IGFBP-3 only when phosphorylated at serine 183.

• References:

  C. Schmid, Am J Physiol Endocrinol Metab, Aug 1994; 267: E226 - E233. Hanh Le, Am J Physiol Endocrinol Metab, May 2006; 290: E952 - E960. C. A. Gibson, J Anim Sci, Jun 1999; 77: 1547 - 1557.

• All Sites:

  Human: Ser183; Mouse: Ser183

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGFBP-3 (Phospho-Ser183) antibody. IGFBP-3 (Phospho-Ser183) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.