• Catalog Number:

  E1A1042A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P61978

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  65 kDa phosphoprotein, CSBP, DC-stretch binding protein, HNRNPK, HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP, Transformation upregulated nuclear protein, hnRNP-K

• Specificity Sensitivity:

  hnRNP K (Phospho-Ser284) antibody detects endogenous levels of hnRNP K only when phosphorylated at serine 284.

• References:

  Matunis M.J., Mol. Cell. Biol. 12:164-171(1992). Dejgaard K., J. Mol. Biol. 236:33-48(1994). Humphray S.J., Nature 429:369-374(2004).

• All Sites:

  Human: Ser284; Mouse: Ser284

Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using hnRNP K (Phospho-Ser284) antibody.