• Catalog Number:

  E1A1040A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HMG14 around the phosphorylation site of serine 21 (R-R-SP-A-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P05114

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  HMG14, High-mobility group nucleosome binding domain 1, Nonhistone chromosomal protein HMG-14

• Specificity Sensitivity:

  HMG14 (Phospho-Ser21) antibody detects endogenous levels of HMG14 only when phosphorylated at serine 21.

• References:

  Mihael H. Polymeropoulos. Nucleic Acids Res., Jul 1991; 19: 3753. LJ Arwood. J. Biol. Chem., Jun 1990; 265: 9771 - 9777. Thomas Hildmann. Genome Res., Apr 1999; 9: 360 - 372.

• All Sites:

  Human: Ser21; Mouse: Ser20; Rat: Ser21

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HMG14 (Phospho-Ser21) antibody. Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) antibody.

HMG14 (Phospho-Ser21) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.