• Catalog Number:

  E1A1028A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDK9 around the phosphorylation site of threonine 186 (R-Y-TP-N-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P50750

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  C-2K; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; cyclin-dependent kinase 9; EC 2.7.11.22; EC 2.7.11.23; kinase Cdk9; PITALRE

• Specificity Sensitivity:

  CDK9 (Phospho-Thr186) antibody detects endogenous levels of CDK9 only when phosphorylated at threonine 186.

• References:

  Grana X., Proc. Natl. Acad. Sci. U.S.A. 91:3834-3838(1994). Best J.L., Biochem. Biophys. Res. Commun. 208-562-568(1995). Liu H., Gene 252:51-59(2000).

• All Sites:

  Human: Thr186; Mouse: Thr186; Rat: Thr186

Western blot analysis of extracts from 3T3 cells, treated with Forskolin (40nM, 30mins), using CDK9 (Phospho-Thr186) antibody.