• Catalog Number:

  E1A1027A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC16/APC6 around the phosphorylation site of serine 560 (I-I-SP-P-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13042

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  APC6, Anaphase promoting complex subunit 6, CDC16Hs, Cell division cycle protein 16 homolog, Cyclosome subunit 6

• Specificity Sensitivity:

  CDC16/APC6 (Phospho-Ser560) antibody detects endogenous levels of CDC16/APC6 only when phosphorylated at serine 560.

• References:

  Tugendreich S., Cell 81:261-268(1995). Ollendorff V., J. Biol. Chem. 272:32011-32018(1997). Kallio M., J. Cell Biol. 141:1393-1406(1998).

• All Sites:

  Human: Ser560; Mouse: Ser560

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC16/APC6 (Phospho-Ser560) antibody. Western blot analysis of extracts from HuvEc cells, using CDC16/APC6 (Phospho-Ser560) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using CDC16/APC6 (Phospho-Ser560) antibody.