• Catalog Number:

  E1A1011A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tryptophan Hydroxylase around the phosphorylation site of serine 58 (R-N-SP-E-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17752

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  EC 1.14.16.4, TPH, TRPH, tryptophan 5-hydroxylase 1, tryptophan 5-monooxygenase 1

• Specificity Sensitivity:

  Tryptophan Hydroxylase (Phospho-Ser58) antibody detects endogenous levels of Tryptophan Hydroxylase only when phosphorylated at serine 58.

• References:

  Ji Ying Sze, Development, Aug 2002; 129: 3901. Jeremy Veenstra-VanderWeele, Mol. Interv., Mar 2003; 3: 72. Jose C. Florez, J Biol Rhythms, Sep 1996; 11: 241 - 257. Nurgul Carkaci-Salli, J. Biol. Chem., Sep 2006; 281: 28105 - 28112.

• All Sites:

  Human: Ser58; Mouse: Ser61; Rat: Ser58

Western blot analysis of extracts from 293 cells, treated with paclitaxel (1uM, 24hours), using Tryptophan Hydroxylase (Phospho-Ser58) antibody.