• Catalog Number:

  E1A1005A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 208 (N-L-SP-P-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (Phospho-Ser208) antibody detects endogenous levels of Smad3 only when phosphorylated at serine 208.

• References:

  Wen Zhang. J. Biol. Chem., Dec 2000; 275: 39237. Brendan D. Looyenga. Mol. Endocrinol., Jul 2007; 10.1210/me.2006-0402. Jian Xu. J Androl, Mar 2003; 24: 192.

• All Sites:

  Human: Ser208; Mouse: Ser208; Rat: Ser208

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Smad3 (Phospho-Ser208) antibody. Western blot analysis of extracts from Jurkat cells, using Smad3 (Phospho-Ser208) antibody.