• Catalog Number:

  E1A1004A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204(A-G-SP-P-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (Phospho-Ser204) antibody detects endogenous levels of Smad3 only when phosphorylated at serine 204.

• References:

  Melhuish T.A., J. Biol. Chem. 276:32109-32114(2001). Wu K., J. Biol. Chem. 278:51673-51684(2003). Wang H., J. Biol. Chem. 280:5154-5162(2005) --

• All Sites:

  Human: Ser204; Mouse: Ser204; Rat: Ser204

Western blot analysis of extracts from NIH/3T3 cells, treated with Serum (20%, 15mins), using Smad3 (Phospho-Ser204) antibody.