• Catalog Number:

  E1A1003A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.

• References:

  Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

• All Sites:

  Human: Thr179; Mouse: Thr179; Rat: Thr179

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho-Thr179) antibody. Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho-Thr179) antibody.