• Catalog Number:

  E1A1001A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad2/3 around the phosphorylation site of threonine 8(P-F-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022/Q15796

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3; JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2

• Specificity Sensitivity:

  Smad2/3 (Phospho-Thr8) antibody detects endogenous levels of Smad2/3 only when phosphorylated at threonine 8.

• References:

  Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

• All Sites:

  Human: Thr8; Mouse: Thr8; Rat: Thr8

Western blot analysis of extracts from RAW264.7 cells, using Smad2/3 (Phospho-Thr8) antibody.